Electrophoretic Comparison between Native Protein Patterns in the Hepatitis C and Human Immunodeficiency Virus Patients

Abstract

Hepatitis C virus is one of the main causes of chronic hepatitis in developing countries. There are 170 million affected people around the world as reported by the World Health Organization. Nowadays we are looking for monitoring the advanced tools during the most recent of the shortage reagent and equipments troubles which are strongly variables. This work studies the evaluation of diagnosis Hepatitis C and human immunodeficiency viruses diseases by Serum Protein Electrophoresis (SPE) as a marker. SPE designing is as to separate serum protein to five fractions (Albumin, Alpha-1, Alpha-2, Beta, and Gamma) to estimate its individual concentration of the five fractions for the viral infected samples. The elevated serum protein, particularly in the gamma-globulin fraction is frequently associated with HIV infection. All the samples were compared to control samples. It was found that the HCV and HIV infections caused irregular alterations in the electrophoretic protein patterns in serum of the HCV and HIV patients. These alterations were represented at the protein level by disappearance of normal protein bands and / or appearance of abnormal unique bands. Native proteins expressed as a result of the infection with HCV and HIV was detected electrophoretically by vertical polyacrylamide gel electrophoresis.