Abstract
1. 1. Hemoglobin phenotypes have been determined for closely related pocket gopher subspecies in the genus Thomomys from sea level and high altitude. 2. 2. A single hemoglobin band was demonstrated for each gopher population with cellulose acetate membrane electrophoresis, with a mobility of 73% relative to human Hb-A. 3. 3. Using polyacrylamide gel electrophoresis, a major Hb component (94% of the total Hb) showed a relative mobility of 68–69%; the minor Hb component showed a relative mobility of 74–75%. 4. 4. The electrophoretic and physiological similarities of the gopher hemoglobins have persisted despite extreme differences in ambient P O 2 in the habitats of each subspecies.
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