Abstract
Abstract1. The gastrocnemius muscles of frog were exposed to an electric field of direct current generated by a gradient of 10 volts D. C. per centimeter for 20 minutes in nitrogen environment.2. Activity of marker enzymes such as succinic, malic and glutamic dehydrogenases to locate mitochondria, xanthine oxidase to locate the supernatent protein fraction, and 1-amino acid oxidase to locate the microsomes was studied in the control and experimental muscle halves.3. Both the structural (buffer insoluble) and soluble (TCA insoluble) protein fractions of the fibres were involved in the electromigration.4. Marker enzyme studies indicated that mitochondria and microsomes had a net positive sign of the charge and migrated to the cathode in an electric field of direct current.5. Activity of enzymes was under the influence of either a net positive or negative sign of the charge on the protein content and possibly on the density of charges.
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More From: Archives internationales de physiologie et de biochimie
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