Abstract
Electrophoretic mobilities of rat B cells and T cells were measured at different pH and ionic strengths. The results were analyzed by a mobility formula which directly relates the measured electrophoretic mobility with the charge density in the surface region of cells. As a result, it was revealed that for both B cells and T cells the distribution of the acidic groups in the surface region is rather uniform, while the density of the basic groups increases inwardly with increasing distance from the outer end of the surface region. In addition, the average dissociation constants of the acidic and basic groups in the surface region were estimated from the charge density as a function of pH for both types of cells and suggested that the surface of B cells is more acidic than that of T cells.
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