Electrophoretic analysis of sarcoplasmic proteins from fish muscle on polyacrylamide gels.

Abstract

Sarcoplasmic proteins were extracted from the ordinary muscle of twelve species of marine and four species of freshwater fish. Disc-gel electrophoresis in the absence of SDS resulted in species-specific patterns. Patterns of marine fishes widely differed from those of freshwater fishes. Polyacrylamide slab gel electrophoresis in the presence of SDS made it possible to categorize those fishes into three groups by patterns: marine white-fleshed, bottom fish (rich in 43K and 35K components), marine red-fleshed, pelagic fish (rich in 43K, 40K and 35K components), and freshwater fish (rich in 43K component). Red sea bream, Pacific mackerel and carp, as repre-sentatives of the three groups, were analyzed for relative amounts of 43K, 40K and 35K com-ponents in the total sarcoplasmic proteins: 12.6, 13.7 and 13.5% in red sea bream, 13.7, 18.7 and 11.6% in Pacific mackerel, and 19.5, 12.3 and 10.7% in carp, respectively.