Abstract
Proteins extracted from different date palm ( Phoenix dactylifera L.) trees “Khalas” Cultivar leaflets, which were collected from 10 different locations of Al-Ah sa oasis in the eastern province of Saudi Arabia, h ave been analyzed by SDS-PAGE electrophoresis. The present study showed different protein patterns, which revealed by NJ and UPGMA clustering methods into three major clusters 1, 2 and 3. Cluster 1 occupies distinct place in the dendrogram that composed of O Y, OM, T and SH locations samples were reflected (monomorphism). Cluster 2 included A and AM locations samples that distinguished each by unique band. While the Cluster 3 included B, G, J and M location s samples which separated singly one by one, since there are no enough sharing bands to cluster them togethe r except they were low in their protein content. Th e results collectively indicated that the difference in clustering patterns is mainly due to genetic var iations. Additionally, cluster 1 location samples may repres ent the proposed original “Khalas” cultivar, while the other tree samples originated from seeds resembled the original cultivar. More genetic and biochemical studies should be conducted to support this conclus ion. However, identification of the original “Khala s” cultivar is important to guaranty the superior char acteristics of cultivated cultivar trees.
Highlights
Date palm (Phoenix dactylifera L., 2n = 2× = 36) is a monocotyledonous and dioeciously species belonging to Arecaceae family (ITIS, 2010; Elmeer et al, 2011)
Proteins extracted from different date palm (Phoenix dactylifera L.) trees “Khalas” Cultivar leaflets, which were collected from 10 different locations of Al-Ahsa oasis in the eastern province of Saudi Arabia, have been analyzed by SDS-PAGE electrophoresis
The present study showed different protein patterns, which revealed by NeighborJoining Method (NJ) and Unweighted Pair Group Method With Arithmetic Average (UPGMA) clustering methods into three major clusters 1, 2 and 3
Summary
Date palm (Phoenix dactylifera L., 2n = 2× = 36) is a monocotyledonous and dioeciously species belonging to Arecaceae family (ITIS, 2010; Elmeer et al, 2011). It widely cultivated in arid regions of the Middle East and North Africa, (Hamza et al, 2011; Al-Yahyai and AlKhanjari, 2008; Khierallah et al, 2011; Elmeer et al, 2011; Jain, 2011; Abul-Soad and Madi, 2010). Protein pattern analysis by gel electrophoresis has been used in higher plants to study various problems in breeding, genetics, taxonomy, physiology, (Leonardo et al, 2010; Chang et al, 2010; Castillejo et al, 2012; Elshibli and Korpelainen, 2008; Davar et al, 2012) as it has been appreciated as a biochemical tool for studying the phylogentic relationships (Al-Yahyai and Al-Khanjari, 2008)
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