Abstract
Native bile samples, dialyzed against double distilled water, fractionated by Sepharose 2B column chromatography, concentrated by ultrafiltration and lyophilized, were solubilized in lysis buffer and studied by two-dimensional electrophoresis (2-DE) with immobilized pH gradients in the first dimension. We counted 70, and 7 spots in 2-DE patterns of bile from patients with cholesterol gallstones and pigment gallstones, respectively.
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