Abstract
The separation of different uronic acids, oligogalacturonic acids and neutral sugars by electrophoresis on silylated glass-fibre paper is described. As electrolytes 0.1 M solutions of barium, zinc and calcium acetate were used. In these solutions the separation of acid constituents was possible without interference from neutral sugars. Depending on the electrolytes, a mixture of galacturonic, mannuronic, glucuronic and guluronic acid was separated within 90–165 min. The partial characterization of acid polysaccharides such as pectin, alginate and mixtures thereof is described.
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