Electrophoresis of proteins from human parotid saliva

Abstract

Polyacrylamide gel electrophoretic patterns of parotid salivary proteins from healthy young adults were obtained with a standard procedure. A uniform pattern showing fourteen anodal bands is found in most of the samples. Repeated studies on the same subjects over a period of more than a year demonstrated a striking constancy of patterns throughout that period. Amylase, the most prominent fraction, studied from eluates of the original gel, revealed a complex of 6 isoenzymes. The patterns described are correlated with those described by other authors using polyacrylamide gel electrophoresis. The importance of the technique as a tool for clinical research and studies of large groups is emphasized.