Electrophoresis of Proteins and Nucleic Acids

Abstract

Abstract Electrophoresis forms the basis of routine separation and analysis of nucleic acid and protein molecules. It is difficult to scan the biological literature without some form of electrophoresis being used in the methods and results for critical preparation and analysis. The applications of nucleic acid electrophoresis include purification, genetic analysis, DNA probe preparation, and DNA and RNA sequence determination. Protein electrophoresis is used for purification, size estimation, subunit composition, protein expression, and identification of posttranslational modifications, in addition to forming the basis for the first step in immunoblotting. While the analysis of proteins and nucleic acids is described in detail, the future applications of microelectrophoresis and microfluidics are also discussed. The bibliography contains both historically significant references and current references outlining established protocols, providing the bench user a guide to the use of electrophoretic techniques in their research.