Abstract
Former results on protein-modified electrofusion of barley protoplasts have been extended by human cell membrane electropermeation and electrofusion after preincubation of cells with proteolytic enzymes. After pulsation, the amount of cells electroporated or broken down increased eight times and also the resealing time was prolonged. That means the lipid bilayer (protein content diminished by lysis) has a lower resistance against permeation. However, the rule of the relative electrofusion yield determination by the protein isoelectric point is still valid. This study of biopolymer interaction with human cell membranes is a prerequisite for drug incorporation (delivery) and electrotransformation by nucleic acids.
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