Electropenetrography Monitoring of the Neotropical Brown-Stink Bug (Hemiptera: Pentatomidae) on Soybean Pods: An Electrical Penetration Graph-Histology Analysis.

Tiago Lucini,Antônio R Panizzi

doi:10.1093/jisesa/iey108

Tiago Lucini, Antônio R Panizzi

Open Access

https://doi.org/10.1093/jisesa/iey108

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Abstract

The Neotropical brown-stink bug Euschistus heros (F.) (Hemiptera: Pentatomidae) is the most important pest damaging soybean in the Neotropics, the world largest production area. The alternating current–direct current (AC–DC) electropenetrography (EPG) technology was used to develop an EPG waveform library of adult females feeding on soybean (Glycine max (L.) Merr.) pods at different input resistor (Ri) levels. Thirteen waveform types/subtypes were identified and described. They were divided into non-probing (Z, Np, Dw1, and Dw2), and probing waveforms (Eh1, Eh2, Eh3, Eh4, and Eh5). Probing waveforms were grouped into three phases: 1) pathway (Eh1a, Eh1b, Eh1c, and Eh1w), 2) ingestion (Eh2, Eh3a, Eh3b, and Eh4), and 3) interruption (Eh5). Correlations between waveforms Eh1b, Eh1c, Eh2, Eh3, and Eh4 and stylets tip position and/or salivary sheath in the pod tissue were determined via histological studies. Non-probing waveforms, Z and Np were visually associated with the bug resting and walking on pod surface, respectively. Waveform Dw1 was correlated with egestion, and the ingestion of fluids (droplets) was proposed for Dw2. Eh1a and Eh1b corresponded to initial and deep stylet penetration through pod tissue, and secretion of a salivary sheath. In Eh1c, stylets penetrated the rigid cell layer of sclerenchyma, and during Eh1w they were withdrawn. Eh2 represents sustained xylem sap ingestion. Eh3a corresponded to lacerate and macerate cell rupture feeding behavior in seed endosperm, whereas Eh3b corresponded to ingestion of cellular contents. Eh4 represented short ingestion from an unknown site, and Eh5 represented short interruptions during xylem sap ingestion.

Highlights

The Neotropical brown-stink bug Euschistus heros (F.) (Hemiptera: Pentatomidae) is the most important pest damaging soybean in the Neotropics, the world largest production area
The alternating current–direct current (AC– DC) electropenetrography (EPG) technology was used to develop an EPG waveform library of adult females feeding on soybean (Glycine max (L.) Merr.) pods at different input resistor (Ri) levels.Thirteen waveform types/subtypes were identified and described
This study aimed to: 1) monitor and characterize an EPG waveform library produced by E. heros adult females feeding on soybean pods at the pod-filling stage (R5) at different Ri levels using the new AC–DC EPG monitor; 2) determine their specific feeding sites and; 3) ascertain the biological meanings of the waveforms based on their electrical characteristics and histological correlations

Summary

Introduction

The Neotropical brown-stink bug Euschistus heros (F.) (Hemiptera: Pentatomidae) is the most important pest damaging soybean in the Neotropics, the world largest production area. In the Neotropics, E. heros has preference to feed on fabaceous plants, mostly on soybean (seed endosperm, its preferred food source) It has been observed on other 20 plant species from 10 different families, with preference, besides Fabaceae, for Solanaceae, Brassicaceae and Asteraceae (Smaniotto and Panizzi 2015). A tool known as electropenetrography (EPG) provides complete and accurate means to evaluate the feeding behavior of any hemipteran (Walker 2000) In this technique, an electrical circuit between the sucking insect and its food is created, and the low electrical current that flows through the system generates waveforms that represent different feeding activities, including stylet penetration, salivation, and ingestion (Tjallingii 1978, Walker 2000). The first electropenetrograph generation was designed using alternating current (AC) applied

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