Electron-microscopic evidence of mitochondriae containing macroautophagy in experimental acute pancreatitis: Implications for cell death

Abstract

BackgroundDysfunctional autophagy and necrosis are characteristic features of severe acute pancreatitis. ObjectiveTo unravel the cellular mechanisms underlying the pathogenesis of acute pancreatitis. MethodsWe studied the ultrastructural pancreatic morphology using electron microscopy in experimental acute pancreatitis. The control group of animals received intraperitoneal injections of normal saline. Different severity of acute pancreatitis was induced by low and high doses of caerulein in Swiss albino mice. In the low dose group, pancreatitis was induced by 4 injections of caerulein given hourly [50 μg/kg/dose – total of 200 μg/kg] and in the high dose group by 8 injections given hourly (total of 400 μg/kg). The experiments were repeated in Na-taurocholate model of acute pancreatitis in rats. The pancreatic tissue was processed and studied by transmission electron microscopy for ultrastructural changes. ResultsThe acinar cells of the pancreatitis animals revealed autophagosomes that contained cellular organelles, including mitochondria. The animals that received a higher dose of caerulein had numerous cells showing a necrotic morphology, whereas the animals in the low dose group showed a predominantly apoptotic cell morphology. The Na-taurocholate model in rats also showed similar features of severe pancreatitis with cellular necrosis and macroautophagy. ConclusionsDysfunctional mitochondria in the injured pancreatic acinar cells are degraded by macroautophagy. These observations are not model specific. Mitochondrial dysfunction and consequent energy deficit in the cells might be causally related to cellular necrosis.