Abstract
We have utilized the Hewlett-Packard LC/ESI-MS technology to store electronic records of total ion current profiles of molecular species of various natural and peroxidized phospholipids of native and auto-oxidized plasma lipoproteins before and after digestion with secretory phospholipase A2 (sPLA2). The present report illustrates the successful utilization of the stored data in several originally un-intended applications, including chemical, physico-chemical and enzymatic alterations of the lipoprotein lipids. The inclusion of lipid samples from enzyme and control incubations adds a dynamic aspect to multidimensional lipidomic analysis of plasma lipoproteins.
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