Abstract
INTRODUCTIONThe current study aimed to investigate the effects of electronic cigarettes on insulin sensibility in ApoE gene knockout mice.METHODSIn total, 48 male ApoE gene knockout mice were randomly divided into four exposure groups: 1) electronic cigarette (e-cigarette) containing 12 mg/mL of nicotine, 2) e-cigarette without nicotine (0mg), 3) traditional cigarette (cigarette), and 4) fresh air (control). The first three groups were exposed to the associated smoke for 18 weeks. The body weight was recorded regularly in the four groups. After the last exposure, the concentrations of lipids, hs-CRP and TNF-α in serum were detected and the effect of electronic cigarettes on insulin tolerance was measured.RESULTSThe levels of serum lipid, hs-CRP and TNF-α in the e-cigarette, 0mg and cigarette groups were significantly increased compared with those in the control group (p<0.05). Also, the insulin tolerance in the e-cigarette, 0mg and cigarette groups was significantly decreased compared to that in the control group (p<0.05).CONCLUSIONSElectronic cigarettes showed comparable effects to traditional cigarettes in influencing the metabolic functions in ApoE gene knockout mice.
Highlights
The current study aimed to investigate the effects of electronic cigarettes on insulin sensibility in ApoE gene knockout mice
The aim of the current study is to explore the effect of e-cigarette exposure on insulin sensibility based on the determination of serum lipids and chronic inflammatory indexes in order to clarify the potential hazards of e-cigarettes
All animal experiments were performed in accordance with the guidelines of the Xi’an Medical University (Shaanxi, China), and were approved by Detection of blood lipids and inflammatory indexes The levels of total cholesterol, triglyceride, lowdensity lipoprotein and high-density lipoprotein were determined by automatic biochemical analyzer
Summary
The current study aimed to investigate the effects of electronic cigarettes on insulin sensibility in ApoE gene knockout mice. METHODS In total, 48 male ApoE gene knockout mice were randomly divided into four exposure groups: 1) electronic cigarette (e-cigarette) containing 12 mg/ mL of nicotine, 2) e-cigarette without nicotine (0mg), 3) traditional cigarette (cigarette), and 4) fresh air (control). ApoE gene knockout mice are frequently used to investigate cardiovascular diseases; it is an ideal animal model to study the diseases related to the changes of blood lipids[6]. The aim of the current study is to explore the effect of e-cigarette exposure on insulin sensibility based on the determination of serum lipids and chronic inflammatory indexes in order to clarify the potential hazards of e-cigarettes
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