Abstract
Abstract The fluorescence spectra of 5-hydroxytryptamine (serotonin) in acidic media have been studied by means of fluorescence polarization, temperature dependence of fluorescence, and kinetics of the protonation in H2O and D2O. The red-shifted emission appearing by protonation in the excited state is ascribed to the protonated form of serotonin at C4. In order to confirm the assignment of electronic spectra, the absorption spectra of the single crystal of 5-methoxyindole-3-acetic acid have been measured by means of reflection technique, the directions of transition moments being determined by polarization analysis. Calculation was carried out on the energy levels of the excited state for serotonin and its protonated forms, and the directions of transition moments were compared with the experimental results.
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