Abstract

Summary Electron transport system (ETS) activity was determined in the estuarine sedimentby measuring the reduction of 2-(-p-iodophenyl)-3-(p-nitrophenyl)-5-phenyl tetrazolium chloride (INT) to iodonitrotetrazolium formazan (INT-formazan) by sediment enzymes and microorganisms. ETS activity was higher in aerobically incubated sediment than in anaerobically incubated sediment. Oxygen uptake in aerobically incubated sediment correlated highly with sediment ETS activity, whereas CO 2 , evolution and nitrogen fixation (C 2 H 2 reduction) correlated very highly with ETS activity in anaerobically incubated sediment.

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