Abstract
The native azurin (Az) was chemically modified with activated poly(ethylene oxide) (PEO) to solubilize in the PEO oligomers. PEO 2000 (average molecular weight of 2000)-modified Az (PEO 2000-Az) was dissolved without denaturation in PEO 200 (average molecular weight of 200). The electronic absorption spectrometry exhibited no detectable conformational change of PEO 2000-Az in phosphate buffer. The electron paramagnetic resonance (EPR) spectrum of PEO 2000-Az is also quite similar to that of native Az in water. This result confirms that the chemical modification with PEO 2000 does not cause any structural change around the copper site. Moreover, since no changes in the EPR spectrum are observed in PEO 200, the copper coordination geometry must be maintained even in PEO 200. The redox activity of PEO 2000-Az in PEO 200 (containing 0.5 mol dm −3 NaClO 4) was investigated by cyclic voltammetry. When we use the PEO 350-SH (α-methoxy-ω-mercapto-poly(ethylene oxide); PEO with average molecular weight of 350) modified gold electrode as a working electrode, the redox response was observed in PEO 200. The result of the measurements for the scan rate dependence suggests that PEO 2000-Az could be immobilized on the PEO 350-SH modified electrode.
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