Electron transfer pathways in microbial oxygen biocathodes

Abstract

The ability of some bacteria to enhance the rate of cathodic oxygen reduction to water has been recently discovered, opening the way to an entirely renewable and environmentally friendly concept of biocathode. In this study we reveal that several mechanisms may induce catalytic effects by bacteria. These comprise mechanisms that are putatively beneficial to the bacteria as well as mechanisms which are merely side effects, including quinone autoxidation and direct O(2) reduction by heme compounds. Here we showed that I mu M of ACNQ is able to generate a significant catalytic wave for oxygen reduction, with onset at approximately 0V vs. SHE. Similarly, adsorption of hemin on a carbon surface catalyses 02 reduction to H(2)O(2) with an onset of +0.2V vs. SHE. To evaluate the catalytic pathways of live cells on cathodic oxygen reduction, two species of electrochemically active bacteria were selected as pure cultures, namely Acinetobacter calcoaceticus and Shewanella putrefaciens. The former appears to exploit a self-excreted redox compound with redox characteristics matching those of pyrroloquinoline quinone (PQQ) for extracellular electron transfer. The latter appears to utilise outer membrane-bound redox compounds. Interaction of quinones and cytochromes with the membrane-bound electron transfer chain is yet to be proven. (C) 2009 Elsevier Ltd. All rights reserved.