Abstract
The generation of 1O 2 1Δ g during autoxidation of tetracyclines (tetracycline, oxytetracycline, chlortetracycline and methacycline) in the presence of Co(II) ions was studied using electron spin resonance (ESR) and spectrophotometry methods. 2,2,6,6-tetramethylpiperidine was used as a trap in the ESR study. The spectrophotometry determination of 1O 2 was based on bleaching of p-nitrosodimethylaniline (RNO) caused by the product of the reaction of 1O 2 with imidazole and was followed by monitoring the decrease in optical density at 440 nm. The effect of 1O 2 quenchers on the concentration of nitroxide radicals and on the bleaching of RNO was studied.
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