Abstract
This paper will introduce electron probe x-ray microanalysis (EPMA) using both energy dispersive (EDS) and wavelength dispersive (WDS) spectrometry and digital EPMA elemental x-ray imaging to the biological researcher who has had little or no experience with these methods. The instrumentation, physics, quantitation and artefacts of these methods will be discussed, compared and contrasted. Biomedical applications of digital WDS-EPMA x-ray imaging of histological sections and quantitative EDS-EPMA of whole cells will be presented. Specifically, Pt distribution in abdominal wall of rats given the drug cisplatin, Ca and Al distributions in human pathological brain tissue, and Mg quantitation of individual peripheral blood mononuclear cells will demonstrate biomedical applications of these methods.
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