Electron Paramagnetic Resonance: Study of the Regulatory Mechanisms of Light Phases of Photosynthesis in Plants

Abstract

The electron paramagnetic resonance (EPR) method is widely used in the biophysics of photosynthesis, because it serves as an effective tool for exploring the processes of electron and proton transport in various photosynthetic systems. This study on the regulation of electron transport in chloroplasts was performed with the direct participation of the authors using the EPR method. The possibilities of the EPR method to study the kinetics of electron transport in chloroplasts of higher plants in situ (leaves of higher plants) at room temperature were shown, and the EPR spectra of chloroplasts at cryogenic temperatures were considered. The latter is of particular importance for substantiating the “kinetic” method of pH measurement inside thylakoids, which was used.