Abstract
This electron microscope investigation of Malassezia furfur (parasitic phase) is basic information for taxonomic purposes. Skin was excised from lesions of tinea versicolor, fixed in paraformaldehyde and osmium tetroxide, and imbedded in Epon. Scales from tinea versicolor were fixed in (a) glutaraldehyde and osmium tetroxide and (b) potassium permanganate, post-fixed in uranyl acetate, and imbedded in Vestopal. Thin sections were stained with uranyl acetate and lead citrate. Filaments and conidia of M. furfur were examined at initial magnifications of 3,000 to 30,000. The walls of the filaments and conidia were thick, fibrous, and sculptured on their inner surfaces. Separation of cells was by fission of a cross wall. In the epidermis M. furfur was observed to lie inside and between the keratinized cells of the stratum corneum.
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