Abstract
Fixation of bacteria and bacteria containing developing intracellular phage with a mixture of glutaraldehyde (2–5%) and uranyl acetate 0.1% gives sections which are equally as good as the conventional fixation in OsO 4 according to Ryter and Kellenberger. The results further suggest that the fixation is more efficient for structures susceptible to lose easily their DNA content. For instance, intracellular lambda is now shown in numbers compatible with physiological data. The shape of the nuclear area of infected bacteria is, however, not well preserved.
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