Electron Microscopy Methods and Protocols

Abstract

General Preparation of Material and Staining of Sections, Heather A. Davies. Negative Staining of Thinly Spread Biological Particulates, J. Robin Harris. Preparation of Thin-Film Frozen-Hydrated/Vitrified Biological Specimens for Cryoelectron Microscopy, J. Robin Harris and Marc Adrian. The Production of Cryosections Through Fixed and Cryoprotected Biological Material and Their Use in Imunocytochemistry, Paul Webster. High-Pressure Freezing for Preservation of High Resolution Fine Structure and Antigenicity for Immunolabeling, Kent McDonald. The Application of LR Gold Resin for Immunogold Labelling, J. R. Thorpe. Low-Temperature Embedding in Acrylic Resins, Pierre Gounon. Quantitative Aspects of Immunogold Labeling in Embedded and Nonembedded Sections, Catherine Rabouille. Microwave Processing Techniques for Electron Microscopy: A Four-Hour Protocol, Rick T. Giberson and Richard S. Demaree, Jr. Electron Microscopic Enzyme Cytochemistry, Nobukazu Araki and Tanenori Hatae. In Situ Molecular Hybridization Techniques for Ultrathin Sections, Jean-Guy Forunier and Francoise Escaig-Haye. Preparation of the Fission Yeast Schizosaccharomyces pombe for Ultrastructural and Immunocytochemical Study, M. A. Nasser Hajibagheri, Kenneth Sawin, Steve Gschmeissner, Ken Blight, and Carol Upton. Preparation of Double/Single-Stranded DNA and RNA Molecules for Electron Microscopy, M. A. Nasser Hajibagheri. Application of Electron Microscopy for Studying Protein-DNA Complexes, Maria Schnos and Ross B. Inman. X-Ray Miocroanalysis Techniques, A. John Morgan, Carole Winters, and Stephen Sturzenbaum. Index.