Electron microscopy in nephropathology

Abstract

Non-neoplastic kidney diseases represent abroad spectrum of diseases. Although their pathogenesis differs, the histological findings may be similar in terms of conventional morphology. Aprecise classification of these diseases is a prerequisite for correct therapy and prognostic assessment. In the diagnostic process, the magnification achieved by electron microscopy is essential and cannot be replaced by any other technique. The most frequent diagnostic questions addressed by ultrastructural studies represent (1)alterations of podocytes (e.g., minimal-change disease), (2)changes of the thickness and structure of the glomerular basement membrane (e.g., diabetic glomerulosclerosis or Alport disease), (3)the presence, characteristics and exact localisation of immune complexes (e.g., membranous glomerulonephritis or lupus nephritis), (4)alterations of endothelial cells and capillaries (e.g., thrombotic microangiopathy) and (5)diseases of the tubular cells (e.g., light-chain nephropathy or toxic effects). Therefore, ultrastructural investigations are-together with conventional microscopy and immunohistochemistry (or immunofluorescence)-an integral part of the so-called triple-diagnostics in routine nephropathology.