Electron microscopic identification of mammillary body terminals in the rats AV thalamic nucleus by means of anterograde transport of HRP. A quantitative comparison with the EM degeneration and EM autoradiographic techniques

Abstract

A comparison has been made between the number of horseradish peroxidase (HRP)-labeled mammillary body terminals in the antero-ventral (AV) nucleus and the number of such terminals labeled with the electron microscopic (EM) autoradiographic [6] and EM degeneration technique [6]. With the anterograde HRP technique less terminals were labeled than with the autoradiographic technique, but more than with the degeneration technique. Furthermore it may be concluded that the anterograde HRP technique is suitable for determining the type of terminal and does not require the long exposure time of the autoradiographic technique.