Abstract
According to literature data, in the conditions of the balance of pro- and anti-inflammatory factors, the development of the infectious-inflammatory process tends to progress and more severe with subsequent transformation into chronic inflammation with corresponding morphofunctional effects. Under such critical conditions, an apoptosis is likely to play a crucial protective role, which can eliminate the excessive accumulation of aggressive inflammatory effects and effectively eliminate them, which in the future prevents the probable transformation into a persistent form. The aim of the work was to study the features of the early dynamic processes of apoptosis in the tissues of the kidneys under simulation conditions in the experiment of acute pyelonephritis and concomitant diabetes mellitus type I and II. The purpose of the study was to study the features of the early dynamic processes of apoptosis in the tissues of the kidneys under simulation conditions in the experiment of acute pyelonephritis and concomitant type I and type II diabetes. The work was performed on 300 adult Wistar rats, which were divided into 4 groups. Fragments of animal’s kidneys were studied and photographed in an electron microscope PEM-100-01. The results showed that after modeling in the animals of pyelonephritis in the nephrons there were no significant ultrastructural changes. The structure of the podocytes of the outer sheet was almost the same as the structure of the podocytes of the control material, and in some cells there were signs of activation of their metabolic activity. In the structure of the podocytes of the inner leaf of the capsule, dystrophic changes of the internal membrane of the mitochondria were established. In kidney medulla, the structure of the glomerulus was more preserved than in the cortical. Pathological changes of the proximal and distal tubular podocytes, as well as interstitial tissue, are more pronounced than the renal glomeruli. Under the modeling of the common model of pyelonephritis and type 1 diabetes, more pronounced morphological changes occur: destructive changes in the endothelial cells of the glomerular capillaries, the homogenization of the structure of the basement membrane occurs and the mesangial tissue is significantly enlarged. In nephrons and tubules of cortex, changes are manifested to a much greater extent than in the kidney medulla. When studying in clinical conditions the pathogenetic features of acute pyelonephritis in conditions of concomitant diabetes mellitus it is expedient to carry out electron microscopic research with the aim of choosing the optimal corrective therapeutic effect and preventing the unfavorable course of infectious and inflammatory process and its transformation into persistent form. EM (electron-microscopic) studies are highly informative in the study of pathological changes and early dynamic processes of apoptosis in renal tissues in the design of acute pyelonephritis and concomitant diabetes mellitus I and II in the experimental conditions.
Highlights
Current scientific evidence suggests the participation of apoptosis - programmable cell death (PCD) in the pathogenesis of a significant number of infectious and inflammatory processes that have an active course and a Electron microscopi c diagnostics of apoptosi s processes under simulation conditions in the experiment of acute...tendency to chronicity [3, 7, 15, 18]
The results showed that after modeling in the animals ofpyelonephritisinthenephronstherewerenosignificantultrastructural changes
At theelectron microscopy (EM) study of thekidney cortex of animals in the control group (A), it was found that nephrons of the cortex have an electron-dense tissue of the Bowman capsule, under which on the basement membrane place epitheliocytes of the outer sheet of the capsule (OSC)
Summary
Current scientific evidence suggests the participation of apoptosis - programmable cell death (PCD) in the pathogenesis of a significant number of infectious and inflammatory processes that have an active course and a Electron microscopi c diagnostics of apoptosi s processes under simulation conditions in the experiment of acute...tendency to chronicity [3, 7, 15, 18]. Literary data testify that in the conditions of a violation of the balance of pro- and anti-inflammatory factors, the development of the infectious-inflammatory process is characterized by a tendency towards progression and a more severe course of inflammation and its transformation into chronic inflammation with severe morphofunctional consequences. It is suggested that under such critical conditions, an apoptosis that can eliminate the excessive accumulation of aggressive inflammatory effects, their effective elimination, which in the future will provide a more favorable course of the inflammatory process and prevent its transformation into a persistent form, plays a decisive protective role [2, 10, 11, 13, 14]. The data of many authors underlines the transience of the process of self-destruction of cells, for completing which is enough for several minutes or hours [1, 2, 4, 10, 16]
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