Abstract
Unfixed rat gastric mucosa was embedded in hydroxypropyl methacrylate using the Pease method of rapid “inert dehydration” in ethylene glycol. Application of acidic phosphotungstic acid (PTA) to thin sections prior to electron microscopic examination showed intense staining of material associated with the walls of the smooth-surfaced tubules, and the surface of apical, intracellular, and basal microvilli in the acid-secreting (oxyntic) cell. Since Pease has shown, using these techniques, that PTA is specific stain for polysaccharides, a polysaccharide coating is identified on the surface projections and on the inner surface of the smooth-surfaced tubular system of the acid-secreting cell of the stomach.
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