Abstract
The effect of monosodium glutamate on lymphoid organs remains insufficiently studied. Also, no less relevant is the issue of correction of changes caused by the action of monosodium glutamate. The aim of the study was to study the electron microscopic changes in the parenchyma of the lymph nodes of rats under the action of monosodium glutamate for six weeks and during correction with melatonin. The experimental study was performed on 66 white male and female rats of reproductive age. The structure of mesenteric lymph nodes of white rats under the conditions of physiological norm at the electron microscopic level was studied in 10 intact animals. Experimental animals were divided into 4 groups, each with 10 animals. The control was 16 white rats, which instead of a high-calorie diet (HCD) received a standard diet of vivarium. HCD was achieved by adding to the diet of monosodium glutamate at a dose of 0.07 g/kg body weight of rats. The dose of melatonin was 10 mg/kg body weight of rats, administered orally daily at the same time in the afternoon. The electron microscopic structure of the mesenteric lymph nodes of male and female rats of reproductive age of the intact and control groups corresponds to the species norm. The study showed that monosodium glutamate causes changes in the parenchyma of the lymph nodes as in alimentary obesity. After six weeks of HCD, the number of apoptically altered lymphocytes increases. That part of lymphocytes, which has no signs of karyorrhexis or karyolysis, has a karyolemma with deep intussusception, the cytoplasm is enlightened, the tubules of the granular endoplasmic reticulum in cells with signs of edema, dilated, mitochondrial ridges swollen, damaged. There are profound destructive changes in the cellular composition of the organ and violations at the level of all parts of the vascular bed. After six weeks of melatonin correction, the number of macrophages and plasma cells decreased, in some lymphocytes the nucleolus is not clearly expressed, the karyolemma is uneven, the cytoplasm is enlightened, the number of osmophilic (fatty) inclusions decreases both in the intercellular space and in the cytoplasm of the cell. Therefore, the introduction of melatonin led to a significant restoration of the structural organization, and hence the function of this organ.
Highlights
Today, more than 2,500 additives are deliberately added to foods to preserve their properties and extend their shelf life
high-calorie diet (HCD) was achieved by adding to the diet of monosodium glutamate at a dose of 0.07 g/kg body weight of rats
The experimental animals were divided into 4 groups: the first group (10 animals), which were fed a high-calorie diet (HCD) for six weeks by adding monosodium glutamate; the second group (10 animals), which were fed HCD for six weeks, transferred to the standard diet of vivarium and melatonin was administered for two weeks; the third group (10 animals) and the fourth group (10 animals) were the same as the previous one, but melatonin was used for four and six weeks, respectively
Summary
More than 2,500 additives are deliberately added to foods to preserve their properties and extend their shelf life. One of the most widely used additives both in Ukraine and around the world is monosodium glutamate [12, 19]. It increases appetite and enhances the taste of foods, which leads to an increase in the amount of food consumed per day, causing a high-calorie diet as in this experiment. Despite its widespread use in the food industry, some questions about its effects on the body remain unanswered [20]. Sodium glutamate is known to be neurotoxic, able to provoke degeneration of neuronal populations, and its effects on the body are accompanied by the development of pathological conditions such as stroke, epilepsy, schizophrenia, anxiety, depression, Parkinson's disease, Alzheimer's disease, Huntinglefton's disease and Lou Gehrig's disease [19]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
