Abstract
Computed axial tomography is a method used to obtain the internal, three-dimensional structure of a specimen from a series of two-dimensional projections. Our interest in chromosomal structures, which cannot be crystalized because of their size and apparent irregularity, led us to the application of tomography to electron microscopic data. Our specimens required observations of a large range of section thicknesses (0.1 μm to.75 μm), various magnifications (from 1 pixel = 7.5 nm to 1 pixel = 150 nm) and different volumes of reconstructed data ranging from 643 pixels3 to 5123 pixels3. The choice of parameters are determined by the size of the internal structure to be described.
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