Abstract

Mitosis in the slime mold Physarum polycephalum occurs within the nuclear membrane. The nucleolus breaks down into fragments which, until late in anaphase, remain identifiable by groups of electron dense granules. The mitotic spindle is formed within the nuclear membrane, and it first appears as a small plaque of fibrous material at a short distance from the surface of the disintegrating nucleolus. No centriole was found. When the daughter plates move toward the poles at anaphase, fragments of spindle fibers and clumps of amorphous material of unknown (nucleolar ?) origin are left in the middle of the interzone. The parts of the nuclear membrane around the interzone between the daughter plates and toward the poles disappear at late anaphase, and the remainder is probably reutilized in the formation of a new nuclear envelope. The nucleolus is formed by coalescence of material which first appears dispersed among the telophase chromosomes.

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