Abstract
As a result of the successful isolation of the water mold, Pythiogeton, on a glucose-yeast medium, a study was made of the effect of the reaction of the medium, and the concentration of glucose, on the rate of growth, production of acidic metabolic products, and utilization of glucose. Pythiogeton, when grown on vitamin-free casein hydrolysate media, was completely heterotrophic for only one vitamin, thiamine, and required ca. 0.5 ,ug. per 0.3 g. glucose for maximum growth. The fungus was able to use sulfate as the sole source of S during growth, provided N was supplied in the form of ammonium or organic compounds. Amino acids present in casein and yeast could not serve as carbon sources, but a number of carbohydrates, including cellulose, supported vigorous growth. The fungus produced acidic products at approximately the same rate from all sugars which were utilized, except lactose. It was concluded that the lower rate of growth and acid production on lactose was limited by its rate of hydrolysis, and that hydrolysis during growth did not take place in the presence of glucose because of a preferential utilization of the hexose.
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