Abstract
The binding of concanavalin A (Con A) to plasma membrane(PM)-and endoplasmic reticulum(ER)-rich fractions from etiolated maize coleoptiles has been investigated using sequential treatment with Con A and mannosyl-ferritin (Man-Fer). PM vesicles were shown to be extensively labelled at their outer surface, indicating the presence of available mannosyl and/or glucosyl residues in this fraction. Ferritin particles presented a clustered distribution. No label was observed on control vesicles stained in the presence α-methyl-D-mannoside (αMM). Membranes of the ER-rich fraction appeared largely devoid of ferritin particles. Combining this electron microscope study with procedures which render the interior of the vesicles accessible to the lactin, it was found that whereas no modification in the label of PM vesicles occurred, ER membranes were extensively labelled on their cisternal face. The results are totally in agreement with biochemical data reported in the preceding paper (Hartmann et al., Plant Sci. Lett., 30 (1983) 227) and suggest that PM and ER vesicles retain their orientation after grinding of coleoptile tissue.
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