Electron Beam-Modified Collagen Type I Fibers: Synthesis and Characterization of Mechanical Response.

Abstract

Ten MeV electron beam treatment facilitates a biomimetic introduction of cross-links in collagenous biopolymer systems, modifying their viscoelastic properties, mechanical stability, and swelling behavior. For reconstituted collagen type I fibers, electron-induced cross-linking opens up new perspectives regarding future biomedical applications in terms of tissue and ligament engineering. We demonstrate how electron irradiation affects stiffness both in low-strain regimes and in postyield regimes of biocompatible reconstituted rat tail collagen type I fibers. Stress-strain tests show a dose-dependent increase in modulus in the nonlinear elastic response, indicating a central role of induced cross-links in mechanical stability. Environmental scanning electron microscopy after fiber rupture reveals aligned distributed collagen fibril domains under the fiber surface for as-prepared fibers, accompanied by a ductile fracture behavior, whereas, in tensile tests imaged by light microscopy after 10 MeV electron treatment, isotropic network topologies are observed until the occurrence of a brittle type of rupture. Based on the biomimicry of the process, these findings might pave the way for a novel type of synthesis of tailored tendon or ligament substitutes.