Electron Beam Irradiated Chitosan elicits enhanced antioxidant properties combating resistance to Purple Blotch Disease (Alternaria porri) in Onion (Allium cepa).

Abstract

Purpose This study was carried out to assess the effect of irradiated chitosan as an elicitor on the biochemical traits associated with resistance to purple blotch disease in onion. Materials and methods Chitosan was electron beam irradiated at 100 kGy dose to obtain low molecular weight chitosan. Irradiated chitosan at 20 and 0.04% concentration and different time intervals was used as a biological elicitor cum antimicrobial agent against purple blotch disease in onion. Field grown onion (Variety Basanvant 780) plants were foliar sprayed with irradiated chitosan and the biochemical responses were monitored using parameters namely chlorophylls, carotenoids, antioxidant enzymes, phenols, and antifungal enzyme β-1,3 Glucanase using standard methods. Results Compared to control treatment, a positive correlation with irradiated chitosan treatment was observed for an increase in β-1,3-glucanase, peroxidase activity, and contents of total phenolics, chlorophylls, and carotenoids, which cumulatively contributed to resistance response against the purple blotch disease. Irradiated chitosan (0.04%) treated onion plants at 30, 45, and 60 DAT showed a higher total phenolics, β-1,3-glucanase activity, and peroxidase activity besides enhanced antioxidant properties. Conclusion The results suggest that irradiated chitosan has elicited resistance responses against purple blotch disease in onion. The increased production of antioxidant metabolites may provide value addition to onion as a food commodity.