Electromyogram spectrum changes during sustained contraction related to proton and diprotonated inorganic phosphate accumulation: a 31P nuclear magnetic resonance study on human calf muscles.

Abstract

The calf muscles of five clinically healthy men were submitted to isometric exercise and examined by 31P nuclear magnetic resonance (NMR) spectroscopy and electromyography (EMG) to evaluate the influence of proton (H+) and diprotonated forms of inorganic phosphate (H2PO4-) accumulation on EMG spectrum changes. The experiments were performed in a supra-conducting magnet (2.35 Tesla, 35-cm effective diameter) using a surface coil (7-cm diameter) positioned against the calf muscles. The EMG surface electrodes were applied on the gastrocnemius medialis muscle and acquisition of both NMR and EMG signals was synchronized. The exercise consisted of a sustained isometric contraction at 70% of the maximal voluntary contraction until exhaustion. A continuous decrease in phosphocreatine content and a large concomitant increase in H2PO4- was observed in the calf muscles of each subject. A significant increase in H+ concentration was also found when considering the whole population but intracellular acidosis was low for two subjects. Moreover, a quasilinear decrease in mean power frequency (MPF) was found during the test. Changes in MPF were correlated with variations in H+ and H2PO4- concentration but a more significant relationship was found when MPF changes were correlated with H2PO4- concentration. An interpretation of EMG spectrum changes in terms of an accumulation of by-products of anaerobic metabolism and an increase in the relative number of activated slow fibres is proposed.