Electrolyzed-reduced water mitigates amyloid beta toxicity via DAF-16 in C. elegans

Abstract

Alzheimer’s disease is an age-related neurodegenerative disease. Extracellular aggregation of amyloid beta is a well-known hallmark of Alzheimer’s disease and believed to be critical for the development of Alzheimer’s disease. Lots of efforts have been put on finding an intervention alleviating amyloid beta toxicity. Electrolyzed-reduced water is generated near cathode by electrolysis of water and shown to have strong anti-oxidant and lifespan-extending activities in C. elegans. Here, we examined the effect of electrolyzed-reduced water on amyloid beta toxicity and investigated underlying cellular mechanism involved. Worms grown in media prepared with electrolyzedreduced water showed delayed amyloid beta-induced paralysis and age-related decline in motility. DAF-16, a FOXO transcription factor modulating insulin/IGF-1-like signal, is required for the protection against amyloid beta toxicity. These findings indicate that electrolyzed-reduced water can prevent amyloid beta toxicity via DAF-16 and suggest that electrolyzedreduced water could be utilized for the development of therapeutic intervention to Alzheimer’s disease.