Electrolyzed-reduced water increases resistance to oxidative stress, fertility, and lifespan via insulin/IGF-1-like signal in C. elegans

Seul-Ki Park,Sang-Kyu Park

doi:10.4067/s0716-97602013000200005

Seul-Ki Park, Sang-Kyu Park

Open Access

https://doi.org/10.4067/s0716-97602013000200005

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Journal: Biological Research	Publication Date: Jan 1, 2013
Citations: 20	License type: cc-by

Affiliation: Soonchunhyang University

Abstract

Electrolyzed-reduced water (ERW) scavenges reactive oxygen species and is a powerful anti-oxidant. A positive correlation between oxidative stress and aging has been proved in many model organisms. In Caenorhabditis elegans, many long-lived mutants showed reduced fertility as a trade off against longevity phenotype. We aimed to study the effect of ERW on oxidative stress, fertility and lifespan of C. elegans. We also investigated the genetic pathway involved in the effect of ERW on resistance to oxidative stress and lifespan. We compared lifespan and fertility of worms in media prepared with distilled water and ERW. ERW significantly extended lifespan and increased the number of progeny produced. Then the effect of ERW on resistance to oxidative stress and lifespan of long-lived mutants was determined. ERW increased resistance to oxidative stress and lifespan of eat-2, a genetic model of dietary restriction, but had no effect on those of age-1, which is involved in insulin/insulin-like growth factor (IGF)-1-like signal. In addition, knockdown of daf-16, the downstream mediator of insulin/IGF-1-like signal, completely prevented the effect of ERW on lifespan. These findings suggest that ERW can extend lifespan without accompanying reduced fertility and modulate resistance to oxidative stress and lifespan via insulin/IGF-1-like signal in C. elegans.

Highlights

An electric current passed through water results in electrolysis, which produces electrolyzed-reduced water (ERW) near the cathode and electrolyzed-oxidized water near the anode
To examine the effect of ERW on the lifespan of C. elegans grown in NGM, we compared the lifespan of wild-type N2 worms grown in liquid NGM prepared with distilled water and ERW
Both mean and maximum lifespan were significantly extended in worms grown in NGM prepared with ERW

Summary

Introduction

An electric current passed through water results in electrolysis, which produces electrolyzed-reduced water (ERW) near the cathode and electrolyzed-oxidized water near the anode. ERW shows positive effects on the local immune response in mice (Lee et al, 2009)

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