Abstract
By means of the electrolytic reduction of diphosphopyridine nucleotide at controlled potential using mercury as the cathode, the author has obtained a product which showed an absorption spectra similar to that of dihydrodiphosphopyridine nucleotide. The optical density of the product at 340 mμ diminished partly with the addition of alcohol-dehydrogenase and acetaldehyde or with that of malic acid-dehydrogenase and oxaloacetate as the oxidizing system. The same absorption band was completely disappeared by the addition of a fraction obtained from mung bean seedlings.
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