Abstract
Abstract Aqueous two-phase extraction techniques have been successfully applied to the purification of enzymes and cells. However, due to their similar physical properties, immiscible aqueous phases do not separate rapidly. A method for enhanced demixing of aqueous two-phase systems in a thermostated vertical electrophoresis column was therefore studied. The effects of the electric field strength, field polarity, temperature, phase composition, and buffer concentration on demixing rates of a polyethylene glycol-maltodextrin (PEG-MDX) system were quantitatively measured. At normal electrical polarity (anode at the top of the column), using a maximum practicable field strength of 26.4 V/cm, the demixing rate was twice that in zero electric field at 25 ± 2°C. With poiarity reversed (anode at the bottom, electric field opposing gravitational settling) at a field of 26.4 V/cm, demixing was 5.5 times as fast as in zero field. Reduction of the temperature from 25 to 14°C caused an increase in demixing rate in th...
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