Electrokinetic chromatography with micellar solutions : Retention behaviour and separation of chlorinated phenols

Abstract

The retention behaviour of chlorinated phenols during electrokinetic chromatography with micellar solutions and open-tube capillary columns was investigated. The capacity factor for each compound of a test mixture was calculated by considering both the effects of micellar solubilization and electrophoresis of the ionized solute, even when a solute was ionized. Under conditions where a solute, a chlorinated phenol, was estimated to be partially ionized, the capacity factor of the solute decreased with an increase of pH, whereas that of an electrically neutral solute was almost constant regardless of the change in pH. All the isomers of chlorinated phenols were completely separated within 18 min by using a 650 × 0.05 mm I.D. fused-silica tube with a 0.07 M sodium dodecyl sulphate solution (pH 7.0).