Abstract
A clear precipitating arc of factor VII antigen appeared by imnunoelectrophoresis using 125I-labelled anti-factor VII antibody incorporated in the Larsell plate to enhance the sensitivity. A plasma sample could be electrophoresed directly on 1%agarose containing 15g rabbit anti-human factor VII antiserum, 0.1% 125I-labelled anti-human factor VII specific IgG and 0.5% polyethyleneglycol 6000, and after washing, autoradiographed at -70°C for 24 hr. A distinct rocket was seen from 45 ul standard reference plasma to 16-fold after autoradiography. Of 4 patients with congenital factor VII deficiency,3 patients had no detectable factor VII antigen, one patient had reduced factor VII antigen.
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