Electrogenerated chemiluminescence biosensor for glucose based on poly(luminol–aniline) nanowires composite modified electrode

Abstract

Poly(luminol–aniline) nanowires composite (PLANC) was synthesized on the surface of graphite electrode by electro-oxidizing the mixture of luminol with aniline in the H 2SO 4 acidic medium. The properties of the nanowires composite were characterized by transmission electron microscopy, electrogenerated chemiluminescence and electrochemical impedance spectroscopy. The investigating results showed that: firstly, while the luminol alone was electro-oxidized on the surface of the graphite electrode with the proposed synthetic method, only the polyluminol film was formed on electrode surface. However, while a suitable amount of the aniline was present in the luminol solution, the PLANC could be formed on the electrode surface and this PLANC presented better ECL properties for H 2O 2 compared with pure polyluminol film; secondly, since the PLANC modified electrode not only provided a larger surface area for higher glucose enzyme loading but also formed the nano-structured interface on the electrode surface to improve the analytical performances of the electrogenerated chemiluminescence (ECL) biosensor for glucose, the resulting ECL biosensor presented good response to glucose and a novel ECL biosensor for glucose was proposed.