Abstract

An electrogenerated chemiluminescence (ECL) biosensor array for the individual and simultaneous detection of multiple biomarkers has been developed at first time on the basis of the aptamer-based biosensor incorporating a versatile signal probe. As principle-of-proof, the acute myocardial infarction (AMI) biomarkers, myoglobin(Myo), cardiac troponin I(cTnI) and cardiac troponin T(cTnT) were chosen as model analytes. The biosensor array was fabricated by self-assembling thiolated specific ssDNA aptamers on the surface of gold electrodes, respectively. After each target analyte was bound to the capture probes and then to each corresponding biotinylated antibody, and finally to the versatile ECL signal probe, ECL signals were recorded using PMT or CCD as a detector. For PMT model, the developed method showed extremely low detection limits for 0.30 ng/mL cTnT, 31 pg/mL Myo, 0.79 pg/mL cTnI. For ECL image model, a biosensor array containing three target biosensors and a control biosensor was found to be highly sensitive, no cross-talking, and accurate towards simultaneous detection of the AMI biomarkers. This work demonstrates that the aptamer-based biosensor array with a versatile signal probe is a promising for ECL simultaneous detection of multi-biomarkers.

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