Abstract
In this study, polytaurine film was formed on a gold surface using repetitive cyclic voltammetry (CV) from taurine and phosphate buffer solution (PBS) and used as an electrochemical nanobiosensor for determination of malondialdehyde (MDA). The electrochemical behaviour of polytaurine modified gold (PT/Au) electrode was studied using CV technique. The Electroanalytical behaviour of MDA was investigated at the PT/Au electrode in (PBS), using differential pulse voltammetry. Finally, the applicability of the method to direct assays of human serum and exhaled breath condensate (EBC) is described, and the results showed that this polymeric sensor is suitable for detecting total serum MDA levels of healthy people because the normal level of total MDA in serum is in the range of 0·78–3·10 mM. The results show that by using the proposed sensor, MDA can be determined with detection limits of 34 and 0·995 nM in human serum and EBC samples, respectively.
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