Electrodeposition of platinum nanosheets on C60 decorated glassy carbon electrode as a stable electrochemical biosensor for simultaneous detection of ascorbic acid, dopamine and uric acid

Abstract

ABSTRACT Platinum nanosheets (Pt NSs) were fabricated on fullerene (C 60 ) decorated glassy carbon electrode (GCE) (denoted as Pt NSs/C 60 /GCE) through a simple potentiostatic deposition method. In the coexistence of ascorbic acid (AA), dopamine (DA) and uric acid (UA), the as-prepared Pt NSs/C 60 /GCE electrode exhibited three well-resolved voltammetric peaks (Δ E AA–DA = 176 mV, Δ E DA–UA = 132 mV, Δ E AA–UA = 308 mV) in the differential pulse voltammetry (DPV) measurements, allowing a simultaneous detection of these biomolecules. There are linear relationships between current intensities and concentrations in the region of 10–1800 μM (AA), 0.5–211.5 μM (DA), and 9.5–1187 μM (UA), and the limits of detection (LOD) (S/N = 3) are down to 0.43 μM, 0.07 μM and 0.63 μM for AA, DA and UA, respectively. The as-prepared Pt NSs/C 60 /GCE electrode displayed a good reproducibility and storage stability and was successfully used for detection of AA, DA and UA in real plasma and urine samples.