Abstract
A novel nanocomposite material based on poly-acridine red (PAR), multi-walled carbon nanotubes (MWCNTs) and chitosan (CS) was used to incorporate hematin onto the surface of a glassy carbon electrode for studying the electron transfer and electrocatalytic activity of hematin. A pair of well defined and quasi-reversible redox peaks, corresponding to the hematin Fe(III)/Fe(II) redox couple, appeared at about −0.272 V (vs. Ag/AgCl). The electron transfer rate constant was estimated to be 5.06 s−1. The results indicated that the MWCNT–CS and PAR composite film enhanced the electron transfer process of hematin. The hematin immobilized in the MWCNT–CS and PAR film exhibited bioelectrocatalytic activity for the reduction of H2O2. The peak current of the biosensor by cyclic voltammetry varied linearly with the concentration of H2O2 from 10.0 to 1000.0 μM and the detection limit was 1.75 μM. The amperometric response of the biosensor varied linearly with the concentration of H2O2 from 1.0 to 10.0 μM and the detection limit was 0.61 μM (S/N = 3). The results indicate that the composite film is a versatile platform for the fabrication of electrochemical biosensors.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
