Electrochemiluminescent emission potential tunable Cu–Zn–In–S/ZnS nanocrystals for multiplex microRNAs potential-resolved detection

Abstract

The development of a multi-target simultaneous detection electrochemiluminescence (ECL) strategy remains a great research interest, however, the limited choice of ECL luminophores is the main limitation holding the field back. In this study, a band gap tunable Cu–Zn–In–S/ZnS nanocrystals (CZIS/ZnS NCs) was synthesized and applied to a potential-resolved ECL detection strategy. By changing the Cu ratio in the precursor, the CZIS/ZnS NCs ECL emission spectrum and emission potential were tuned from 480 to 750 nm and −2.10 to −1.11 V, respectively. In addition, an ECL biosensor was fabricated with CZIS/ZnS NCs as signal reporters to detect three types of microRNAs, which could sensitively and selectively determine microRNA-21, microRNA-141, and microRNA-155 in a single cathodic ECL process. The results showed that the ECL intensity was directly linear to the logarithm of the concentration of microRNA-21, microRNA-141, and microRNA-155 from 0.00001 to 10 nM with detection limits of 2.2, 2.6, and 2.7 fM, respectively. This study demonstrates that the emission potential tunable CZIS/ZnS NCs can be employed as a promising alternative to the toxic II-V and II-V NCs to simultaneously detect multiple disease markers, and can guide the further rational design of both ECL luminophores and multi-analyte ECL sensors.