Abstract
A method is presented for electrochemiluminescent (ECL) detection of the food additive curcumin via an energy transfer strategy and by using luminol-doped silica nanoparticles (luminol-NPs). The ECL emission of the luminol-NPs (peaking at 425nm) is reduced in the presence of curcumin due to spectral overlap. The assay can be performed within 1min, response is linear in the 0.1 to 100µM curcumin concentration range, and the limit of detection is 32nM. The method is selective over many ions, adenosine triphosphate, ascorbic acid, cysteine and folic acid. It was successfully applied to the determination of curcumin in spiked human serum and urine. The average recoveries range from 99.0 to 102.6%. Graphical abstract Electrochemiluminescence (ECL) "turn-off" detection of curcumin at levelsas low as 32nM via energy transfer using luminol-doped silica nanoparticles. No hydrogen peroxide (H2O2) is used in ECL detection which makes theluminol-NPs ECL system more stable than the conventional luminol-H2O2 ECL system.
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